assay characteristics performance characteristics alport mastr a simple and robust molecular research assay for the identification of snvs associated with alport syndrome (as) *only 192 mid combinations available. research application ? identification of snvs in the col4a3 , col4a4 and col4a5 genes genes col4a3, col4a4, col4a5 genomic region analyzed 39.4 kb number of amplicons 149 amplicon length 270 - 510 bp number of plexes 4 dna amount required 20 ng per multiplex reaction verifed with lllumina miseq ? sequencing system illumina miseq ? reagent kit flow cell nano v 2 2 x 250 bp v2 2 x 250 bp v3 2 x 300 bp for snvs minimal coverage per allele: 20 23 345* 633* advised maximum number of samples per run:
? agilent technologies, inc. 2017 printed in belgium, december 31, 2017 5991-8401en pr7000-1418 order info for research use only. not for use in diagnostic procedures. multiplicom n.v. [ a ] galile?laan 18, b-2845 niel, belgium [ w ] www.agilent.com [ e ] mtp.customerservice@agilent.com [ t ] +32 3 289 14 00 [ f ] +32 3 400 23 16 cat. no. product name product type reactions mr-0050.008 alport mastr research 4 mid (molecular identifiers) kits are necessary to complete the workflow. workflow plan run multiplex pcr quality control universal pcr quality control equimolar pooling mixing plexes of single sample puri? cation multiplex pcr dilution universal pcr mixing clean up pooling sequencing data analysis mastr work ow ngs
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